Reaction between Reduced Diphosphopyridine Nucleotide and Glutamate Oxalacetate Transaminases
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چکیده
منابع مشابه
Automated assay of activities of enzymes involving the diphosphopyridine nucleotide-reduced diphosphopyridine nucleotide reaction.
A continuous flow instrumental system of analysis, first described by Skeggs (l), was previously applied to the determination of phosphohexose isomerase activity and resulted in a method that was approximately eightfold as rapid as the manual procedure (2). The present paper is concerned with the application of this system of analysis to the determination of enzyme activities that utilize the d...
متن کاملEnzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.
DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myoki...
متن کاملHydrogen Transfer between Reduced Diphosphopyridine Nucleotide Dehydrogenase and the Respiratory Chain. Ii. an Initial Lag in the Oxidation of Reduced Diphosphopyridine Nucleotide.
During studies of the reduced diphosphopyridine nucleotide oxidase activity of various heart muscle preparations, it was observed that some preparations showed a pronounced time lag before attaining the maximal rate of DPNH oxidation. Since this time lag was most apparent with Keilin-Hartree type of preparations from horse heart, a series of studies was carried out to determine the cause of thi...
متن کاملPurification and properties of reduced diphosphopyridine nucleotide oxidase from Azotobacter.
Few studies on purified bacterial reduced diphosphopyridine nucleotide (DPNH) or cytochrome oxidases have been reported, although cytochrome reduction by DPNH’ in crude bacterial extracts has repeatedly been described (l-6). Dolin (7, 8) purified a flavoprotein DPNH oxidase and peroxidase from Streptococcus jaecalis, and Lenhoff and Kaplan (9) and Lenhoff et al. (10) described the characteristi...
متن کاملSuccinic and reduced diphosphopyridine nucleotide oxidase systems of Ascaris muscle.
Although Ascaris lumbricoides, an adult intestinal nematode of the pig, lives in a habitat in which the oxygen tension is extremely low, considerable amounts of oxygen are taken up if the parasite is exposed to an aerobic environment (1, 2). Experiments with intact worms, homogenates, and particulate preparations confirmed these observations and demonstrated that oxygen uptake is dependent mark...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44562-6